Figure 1. Expression of EGFR in pancreas of ElasK-Ras^G12V mice and of patients with pancreatitis and PDAC.

(A) Serial paraffin sections obtained from ElasK-Ras^G12V mice not exposed to doxycycline depicting normal acini, acinar to ductal metaplasia, PanIN1 and PDAC were stained with hematoxylin and eosin (H&E) or with antibodies against the EGFR (EGFR IHC).

Lesions are indicated by solid arrowheads.

Asterisk indicates stroma cells positive for EGFR immunostaining.

Scale bars represent 20 μm.

(B) Serial paraffin sections obtained from ElasK-Ras^G12V mice exposed to doxycycline from conception to P60 and to caerulein from P90 to P180 depicting acini, acinar to ductal metaplasia, PanIN2 and PDAC were stained with H&E or with antibodies against the EGFR (EGFR IHC).

Lesions are indicated by solid arrowheads. Open arrowheads indicate less-differentiated glands within a PDAC.

Asterisk indicates stroma cells positive for EGFR expression.

Scale bars represent 20 μm.

(C) EGFR IHC of human pancreatic biopsies depicting normal pancreata, pancreata from patients with pancreatitis, PanIN lesions (PanIN1 and PanIN3), non metastatic PDAC and a metastatic lymph node with amplified detail.

Lesions are indicated by solid arrowheads. Open arrowheads indicate less-differentiated glands within the metastatic PDAC.

Scale bars represent 50 μm.

See also Figure S1