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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Cell Calcium. 2013 Feb 22;53(4):297–301. doi: 10.1016/j.ceca.2013.01.004

Fig. 1. Fibroblasts respond to purinergic agonists with intracellular Ca2+ increase consistent with expression of P2yR2, P2yR4, P2yR1, and P2xR7.

Fig. 1

(A) Experimental model. Fibroblast cells express purinergic receptors and their activation triggers intracellular Ca2+ signaling. Consequently, disassembly of polymerized actin, manifested as a transient loss of stress fibers. The stress fibers are eventually re-assembled. (B) Ca2+ increase by ATP (100μM) application. N = 8, repeated measures ANOVA with Dunnett test compared to 0 min. (C) Responses of fibroblast Ca2+ by purinergic agonists, ATPγS (100 μM), αβ-meATP (100 μM), ADP (100 μM), BzATP (10 μM), UTP (100 μM), and CCPA (10 μM). N = 6-7. *, p < 0.05, **, p < 0.01, ANOVA with Tukey-Kramer test compared to Control. (D) Purinergic stimulation initiates temporal stress fiber rearrangement. F-actins of the fibroblasts stained with phalloidin (red) before, 10 min after, and 30 min after application of ATP (100 μM). White arrows indicate F-actins that are not along the primary axis of the cells. Scale bars, 30 μm. (E) Summary histograms of actin disassembly over time by ATP (100 μM). N = 4-8. *, p < 0.05, ANOVA with Tukey-Kramer test compared to 0 min.