Figure 3. Labeling Pattern of RhSym4 and RhSym4^{mmp → Ala}.

Labeling of intact parasites with RhSym4 and RhSym4^{mmp → Ala}. Purified schizonts were pretreated with 10 µM of the nonfluorescent compounds Sym4, ≡Sym4, Sym4^{mmp → Ala}, ≡Sym4^{mmp → Ala}, or DMSO. This was followed by 1 hr incubation with 10 µM RhSym4 or RhSym4^{mmp → Ala}. The labeling reaction was stopped by boiling the samples in gel electrophoresis loading buffer. Proteins were resolved by SDS-PAGE and fluorescent proteins were visualized with a flatbed fluorescence scanner. The figure was assembled using two regions of the same gel. Lanes irrelevant to the conclusion were omitted (indicated by white box).

See also Figures S3 and S4.