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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Nat Methods. 2013 Feb 10;10(3):259–264. doi: 10.1038/nmeth.2368

Figure 2. Gel-based profiling of sterol-binding proteins in HeLa cells.

Figure 2

(a) Scheme for treatment of live cells with sterol probes and competitive treatments. (b) HeLa cells treated with 10 μM trans-sterol probe, with and without 365 UV irradiation before click chemistry and SDS-PAGE analysis. (c) Concentration-dependent labeling of live HeLa cells with each probe (cis, epi, trans) at 1, 5, 10, and 20 μM. (d) Competition of sterol probe labeling profiles (10 μM probes) with increasing cholesterol from 0, 10 (1×), 50 (5×), and 100 μM (10×). Fluorescence gel images shown in grayscale.