Table 3. Putative biomarkers of radiation injury of GI tissue of CD2F1 mice (Day 4).
The features that were significantly altered in the irradiated GI tissue after 4 days of IR exposure are listed. These metabolites were validated using tandem mass spectrometry.
| S. No | Metabolites | Kegg/PubChem/Lipid ID | ESI Mode | m/z* | RT* | *Fold Change
|
Major CID fragments | |
|---|---|---|---|---|---|---|---|---|
| 4 Gy | 8 Gy | |||||||
| P-value | P-value | |||||||
| 1 | Spermidine | C00315 | POS | 146.166 | 0.2437 | ↑(0.02) | ↑(0.03) | 72.0819, 112.1130, 84.0822 |
| 2 | Eicosenoic acid | 6438157 | POS | 311.169 | 0.1542 | ↑(0.04) | ↑(0.02) | 183.0119, 119.0515, 79.9594 |
| 3 | Taurocholic acid | C05122 | NEG | 514.285 | 3.1882 | ↑(0.03) | ↑(0.01) | 124.0081, 79.9595, 496.2741 |
| 4 | UDP-N-Acetyl-glucosamine | C00043 | NEG | 606.077 | 0.3992 | ↓(0.02) | ↓(0.03) | 384.9803, 158.9243,78.9694 |
| 5 | PE(O-20:0/20:5) | LMGP02020084 | NEG | 778.574 | 5.0338 | ↓(0.04) | ↓(0.02) | 742.5486 |
| 6 | PI(18:2/16:0) | LMGP06010847 | NEG | 833.534 | 4.2999 | ↑(0.03) | ↑(0.01) | 241.0149, 255.2363, 279.237 |
| 7 | PI(18:1/18:1) | LMGP06010002 | NEG | 861.565 | 4.7912 | ↓(0.02) | ↓(0.05) | 241.0153, 281.2524 |
| 8 | PE(18:2/0:0) | LMGP02050011 | NEG | 476.287 | 1.0965 | ↓(0.01) | ↓(0.05) | 279.2378 |
| 9 | PI(18:0/0:0) | LMGP06050004 | NEG | 599.331 | 1.4042 | ↓(0.02) | ↓(0.04) | 283.2679, 437.2751, 599.3305 |