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. 2012 Nov 13;20(24):26806–26827. doi: 10.1364/OE.20.026806

Table 3. Comparison of Three-color FRET with Two-color Controls.

τfluo
(ns) τCy3
(ns) τCy5
(ns)      Pfluo P Cy3 Dfluo-Cy3 (nm) DCy3-Cy5 (nm)
Three-color 1.2 ± 0.1 0.8 ± 0.1 1.8 ± 0.1 0.85 ± 0.02 0.80 ± 0.04 4.9 ± 0.1 5.2 ± 0.1
Fluo-Cy3 1.1 ± 0.1 1.5 ± 0.1 0.83 ± 0.02 4.8 ± 0.1
Cy3-Cy5 0.8 ± 0.1 1.8 ± 0.1 0.84 ± 0.05 5.2 ± 0.1
Fluo-Cy5 3.9 ± 0.1 1.8 ± 0.1
Single label 3.9 ± 0.1 1.5 ± 0.1 1.8 ± 0.1