Table 3. Comparison of Three-color FRET with Two-color Controls.
τfluo (ns) | τCy3 (ns) | τCy5 (ns) | Pfluo | P Cy3 | Dfluo-Cy3 (nm) | DCy3-Cy5 (nm) | |
---|---|---|---|---|---|---|---|
Three-color | 1.2 ± 0.1 | 0.8 ± 0.1 | 1.8 ± 0.1 | 0.85 ± 0.02 | 0.80 ± 0.04 | 4.9 ± 0.1 | 5.2 ± 0.1 |
Fluo-Cy3 | 1.1 ± 0.1 | 1.5 ± 0.1 | 0.83 ± 0.02 | 4.8 ± 0.1 | |||
Cy3-Cy5 | 0.8 ± 0.1 | 1.8 ± 0.1 | 0.84 ± 0.05 | 5.2 ± 0.1 | |||
Fluo-Cy5 | 3.9 ± 0.1 | 1.8 ± 0.1 | |||||
Single label | 3.9 ± 0.1 | 1.5 ± 0.1 | 1.8 ± 0.1 |