Table 2.
List of genes analyzed for assessing gene expression through RT-qPCR.
| Metabolic pathway | Gene | Protein | Function |
|---|---|---|---|
| PHOTOSYNTHESIS | |||
| PSII | psbA | D1 | Photosystem II 32kDa thylakoid membrane protein. |
| One of the two reaction center proteins of photosystem II | |||
| PSII | psbD | D2 | Photosystem II 34kDa protein. One of the two reaction center proteins of photosystem II, it is needed for assembly of a stable PSII complex |
| PSII | PsbS | CP22 | Photosystem II 22kDa protein, chloroplastic. |
| Seems to be involved in non-photochemical quenching (NPQ) | |||
| PSI | PsaJ | PSI-J | Photosystem I reaction center subunit IX. |
| May help in the organization of the psaE and psaF subunits | |||
| PSI | PsaG | PSI-G | Photosystem I reaction center subunit V, chloroplastic. |
| Function not known | |||
| LHCI | CAB-6A | LHCI type I CAB-6A | Chlorophyll a-b binding protein, chloroplastic. The light-harvesting complex (LHC) captures and delivers excitation energy to associated photosystems |
| LHCI | LHCA4 | LHCI type III CAB-4 | Chlorophyll a-b binding protein, chloroplastic. |
| LHCII | CAB-151 | LHCII type II CAB-151 | Chlorophyll a-b binding protein, chloroplastic. |
| LHCII | LHCB4.2 | LHCB4.2 | Chlorophyll a-b binding protein, chloroplastic. |
| Chloroplastic electron transport chain | SEND33 | Ferredoxin-1 | Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions |
| Carbon dioxide fixation | SSU5B | RuBisCO small chain 5B | RuBisCO catalyzes the carboxylation of D-ribulose-1,5-bisphosphate and the oxidative fragmentation of the pentose substrate |
| Xantophyll cycle -photoprotection | ZEP | Zeaxanthinepoxidase | Zeaxanthin epoxidase plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis |
| RESPIRATION | |||
| Ubiquinol-cytochrome c reductase complex | FES1 | Ubiquinol-cytochrome c reductase iron-sulfur subunit | Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis |
| Mitochondrial electron transport chain | COX5B | Cytochrome c oxidase subunit 5B | One of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport |
| Mitochondrial electron transport chain | AOX1A | Alternative oxidase1A | Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water. Increases respiration when the cytochrome respiratory pathway is restricted |
| Mitochondrial electron transport chain | SDH2-2 | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2 | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH), involved in complex II of the mitochondrial electron transport chain and responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) |
| Tricarboxylic acid cycle | CMDH | Malate dehydrogenase | Catalytic activity: (S)-malate + NAD + = oxaloacetate + NADH |
Both photosynthetic and respiratory genes are shown. Gene names based on best scoring hits obtained blasting sequences against the Swiss-Prot database.