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. Author manuscript; available in PMC: 2013 Nov 8.
Published in final edited form as: Nature. 2012 Nov 8;491(7423):254–258. doi: 10.1038/nature11465

Figure 3. Colorectal tumours exhibit increased permeability to bacteria and their products.

Figure 3

a, Colon segments of CPC-APC and control mice were clipped as indicated and their lumens injected with FITC-dextran or Alexa488-LPS. FITC fluorescence was measured in plasma 1 h later (n = 7, P = 0.017). b, Frozen colon sections prepared 30 min after Alexa488-LPS injection were stained with F4/80 antibody and DAPI and examined by fluorescent microscopy. c, Endotoxin was measured in portal blood of naive or tumour-bearing CPC-APC mice by Limulus bioassay (n = 9, P = 0.066). d–f, Colon sections from CPC-APC mice containing tumours (d) and early lesions (aberrant crypt foci) (e), and early human adenomas (f) were subjected to fluorescent in situ hybridization with eubacterial 16S-rRNA-specific Alexa594-labelled probe and stained with DAPI. Signals are indicated by the arrows. Data represent averages ± s.e.m. *P < 0.05. Scale bars, 50μm.