Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Steroids. 2012 Apr 5;77(7):765–773. doi: 10.1016/j.steroids.2012.03.013

Figure 4.

Figure 4

(A) Kaempferol, apigenin and naringenin, dose-dependently activate PRE-luciferase. Human endometrial stromal cells (HESC) cells were transiently transfected with PRE-luciferase and treated with increasing concentrations of pure compounds. Results are the means of relative light units normalized to β-gal in three independent experiments ± SEM. Significant differences from the control DMSO value were determined by t-test. *p<0.05. (B) Kaempferol, apigenin and naringenin activate PRE-luciferase and can be antagonized by RU486 in human endometrial stromal cells (HESC). HESC were transiently transfected with PRE-luciferase and treated with pure compounds (20 μM) with and without PR antagonist (RU486) for 48 hours. Data represent the average of three independent experiments ± SEM fold change of relative light units normalized to β-gal in triplicate experiments. “a” indicates significant luciferase induction compared to basal DMSO; “b” indicates significant downregulation of luciferase induction by 1 μM RU486. Significant differences from the control DMSO value or the treatment without antagonist were determined by t-test. *p<0.05.