Skip to main content
. Author manuscript; available in PMC: 2014 Jan 25.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Dec 19;430(4):1283–1288. doi: 10.1016/j.bbrc.2012.11.136

Figure 2. Starvation-induced CHOP mediates PUMA and Bim elevation.

Figure 2

A)–C) Cells were incubated in the presence or absence of glutamine with or without media acidification for 10–12 hours and harvested for A) RT-PCR or B) Western. C) Densitometry for PUMA protein relative to Actin for B). D) Cells were electroporated with siRNA either non-targeting or CHOP specific, allowed to recover overnight and treated as in A) then harvested for immunoblot analysis.