Fig. 3.

Increased free radical formation in hepatic tissue of obese mice. (A) Mice were treated as for Fig. 2 and liver tissue was stained for DMPO–protein radicals using a primary rabbit polyclonal anti-DMPO Ab. (B) MFI and nuclei/field were quantified using MetaMorph software and background from saline-treated mice was subtracted from both NC and HF images. (C) Representative Western blot of liver tissues from HF and NC mice. (D) Densitometric analysis of independent blots using total staining for the entire lane. *p < 0.05 vs NC (n=6). For (C) and (D), (−) indicates tissue from age-matched normal chow mice injected with 0.9% NaCl.