Figure 4. Sox10 protein is preferentially upregulated in neural crest cells that differentiate into microvillous neurons.
(A)–(B') Sox10:eGFP+ differentiating microvillous neurons in fixed embryos stained with anti-GFP antibody colocalize with anti-Sox10 antibody staining at 26 hpf (A and A') and 34 hpf (B and B'). Levels of eGFP and Sox10 mirror each other, both increasing as cells ingress and become neurons. Arrowhead in (B and B') indicates a nasal cavity cell likely about to ingress that has increased Sox10 protein expression. Sox10:eGFP: green; Sox10 protein: red; nuclear stain: blue; LOE: left olfactory epithelium. Orientation arrows: D: dorsal; V: ventral; L: lateral. z = 2.5 μm. Scale bars: 15 μm (A–B); 30 μm (C).