Figure 6. LIN-12/Notch signaling instructs vm2 muscle arm by regulating unc-40/DCC and madd-2.
(A) Representative images showing the vulval muscle morphology in wild type (left), unc-40(e271) (middle) and madd-2(ok2226) (right) animals. Boxed areas indicate synaptic regions. Note that unc-40(e271) and madd-2(ok2226) show missing (middle) or abnormal (right) muscle arm phenotypes. Scale bar is 20 μm. (B) Quantification of muscle arm phenotypes in animals with different genotypes indicated on the X-axis. ‘Wild type’ indicates the animals with normal muscle arms. ‘Missing arms’ indicates the animals with missing muscle arms. ‘Abnormal arms’ indicates the animals with flimsy muscle arms or abnormal muscle arms extending from incorrect positions. ‘Other’ indicates animals with severe vulval muscle morphology phenotype that the muscle arms could not be scored. *p<0.0001, chi-squared test, n = 73–194. (C) Double-labeling of the expression of UNC-40 and vulval muscle morphology in wild type (top) and lin-12(wy750) animals (bottom). Left column shows the vulval muscles labeled by mCD8::mCherry. Middle column shows the UNC-40::GFP expression patterns. Right column shows the overlaid images. Note that UNC-40::GFP is preferentially expressed in vm2 and enriched on the muscle arms in the wild type, and is down-regulated in the lin-12(wy750) mutant. Scale bar is 20 μm. (D) Double-labeling of the expression of MADD-2 and vulval muscle morphology in wild type (top) and apx-1(wy755) animals (bottom). Left column shows the vulval muscles labeled by mCD8::mCherry. Middle column shows the MADD-2::GFP expression patterns. Right column shows the overlaid images. Note that MADD-2::GFP is preferentially expressed in vm2 in the wild type, and is down-regulated in the apx-1 (wy755) mutant. Scale bar is 20 μm.
Figure 6—figure supplement 1. LIN-12/Notch signaling instructs vm2 muscle arm by regulating unc-40/DCC and madd-2.
