Video 1.

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DOI: 10.7554/eLife.00378.012

Synchronous Ca²⁺ transients in the vulval muscles of wild-type animals drive egg-laying behavior. Intensity-modulated ratiometric imaging of wild-type C. elegans expressing the Ca²⁺ sensor GCaMP3 and soluble mCherry in the vulval muscles from the unc-103e promoter at 20 fps. Change in the GCaMP3 to mCherry fluorescence ratio is indicated by a rainbow scale from 0 (dark blue) to 1.2 (red). Egg-laying events are observed at 23 and 56 s. Still images from this video are shown in Figure 2C, and the traces of ΔR/R and vulval muscle area are shown in Figure 2E.

DOI: http://dx.doi.org/10.7554/eLife.00378.012