Video 2.

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DOI: 10.7554/eLife.00378.013

lin-12 mutants have asynchronous vulval muscle Ca²⁺ transients leading to uncoordinated contractions. Intensity-modulated ratiometric imaging of lin-12(wy750) mutants expressing the Ca²⁺ sensor GCaMP3 and soluble mCherry in the vulval muscles from the unc-103e promoter at 20 fps. Change in the GCaMP3 to mCherry fluorescence ratio is indicated by a rainbow scale from 0 (dark blue) to 1.2 (red). Still images from this video are shown in Figure 2D, and the traces of ΔR/R and vulval muscle area are shown in Figure 2F.

DOI: http://dx.doi.org/10.7554/eLife.00378.013