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DOI: 10.7554/eLife.00378.014

Asynchronous vulval muscle Ca²⁺ transients in lin-12 and apx-1 mutants can still lead to uncoordinated vulval opening and egg laying. Intensity-modulated ratiometric imaging of apx-1(wy755) mutants expressing the Ca²⁺ sensor GCaMP3 and soluble mCherry in the vulval muscles from the unc-103e promoter at 20 fps. Change in the GCaMP3 to mCherry fluorescence ratio is indicated by a rainbow scale from 0 (dark blue) to 1.2 (red). At 6 and 18 s, there was sufficient vulval opening to permit an egg-laying event.

DOI: http://dx.doi.org/10.7554/eLife.00378.014