Table 1.
BS-Seq samples analyzed in this study
| Sample | Description | Raw reads | Uniquely mapping reads | Coverage (X) | CG error rate | CHG error rate | CHH error rate |
| 1 | WT2003 | 231568902 | 100572780 | 13.5178 | 0.0176 | 0.0122 | 0.0099 |
| 2 | WT2007 | 203541357 | 104376988 | 14.0292 | 0.0107 | 0.0087 | 0.0082 |
| 3 | WT2011 | 187803109 | 84301904 | 11.3309 | 0.0158 | 0.0095 | 0.0065 |
| 4 | T2-PiZt-11-R | 229650259 | 118710094 | 15.9557 | 0.0139 | 0.0099 | 0.0069 |
| 5 | T2-PiZt-11-S | 263329602 | 136471411 | 18.3429 | 0.0101 | 0.0096 | 0.0076 |
| 6 | T4-PiZt-11-R | 270670871 | 131056700 | 17.6151 | 0.0117 | 0.0100 | 0.0074 |
| 7 | T4-PiZt-11-S | 252150298 | 128467721 | 17.2672 | 0.0096 | 0.0076 | 0.0074 |
| 8 | T6-PiZt-11-R | 237280137 | 121966745 | 16.3934 | 0.0105 | 0.0096 | 0.0064 |
| 9 | T6-Pi9-R | 204752699 | 86995742 | 11.6930 | 0.0106 | 0.0093 | 0.0050 |
| 10 | T6-Spin1i-1-R | 215451022 | 90468236 | 12.1597 | 0.0113 | 0.0088 | 0.0061 |
| 11 | T2-PiZt-839-8-R (non functional PiZt) | 238730281 | 117471332 | 15.7892 | 0.0129 | 0.0079 | 0.0056 |
| 12 | T2-PiZt-839-8-S (non functional PiZt) | 211006119 | 106172872 | 14.2705 | 0.0178 | 0.0129 | 0.0095 |
| 13 | WT Callus 1 | 217121522 | 96145279 | 12.9228 | 0.0185 | 0.0178 | 0.0070 |
| 14 | WT Callus 2 | 199261493 | 82617643 | 11.1045 | 0.0232 | 0.0222 | 0.0084 |
| 15 | WT regenerated from tissue culture 1 | 218008835 | 116367626 | 15.6408 | 0.0170 | 0.0155 | 0.0078 |
| 16 | WT regenerated from tissue culture 2 | 225202113 | 97905142 | 13.1593 | 0.0262 | 0.0206 | 0.0093 |
| 17 | WT regenerated from tissue culture 3 | 252306428 | 106544735 | 14.3205 | 0.0194 | 0.0160 | 0.0073 |
| 18 | WT2011 (replicate) | 253971827 | 118140062 | 15.8790 | 0.0172 | 0.0148 | 0.0086 |
Number of raw sequencing reads, number of uniquely mapping reads (post-removal of identical reads), genome coverage (rice genome size = 372 Mb), and error rates are listed. DNA methylation levels of the chloroplast genome were used to estimate error rates. Samples 1–12 and samples 13–18 were prepared separately. “R” and “S” correspond to plants that either contain the transgene (R) or plants in which the transgene was segregated away (S).