Figure 8. Effect of SAM supplementation on histone methylation.
A. Immunostaining for histone H3K4me3 in GV oocytes from animals receiving a control or zinc deficient diet (ZDD) and cultured in medium alone (Control and ZDD) or medium containing 100 µM SAM (ZDD+SAM) for 24 hours. *Significant difference by ANOVA followed by Tukey’s HSD test, P<0.05, N=3 replicates with 10–15 oocytes/group. Scale bar = 50 µm.