Effects of a high protein diet on Drosophila metabolism. Following eclosion, adult flies were raised for ten days on a diet of sucrose (15%, all contents w/V) and differing amounts of yeast extract as indicated. Flies were harvested, weighed and their glucose, glycogen and trehalose levels determined in whole body extracts in at least triplicate. Flies show weight gain with increased food content when on non-starvation diets (1-way ANOVA p < 0.0001; with post-test for linear trend p = 0.0023; asterisk: t-test for the weight drop between 15% and 30%: p = 0.0038). Glycogen and trehalose levels decline with increasing food content (1-way ANOVA with post-test for linear trend: p < 0.0001 and p = 0.0108, respectively), but no changes in glucose levels are observed. Shown is a representative of two independent experiments.