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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Apr 20;1822(8):1230–1237. doi: 10.1016/j.bbadis.2012.04.012

Fig. 3.

Fig. 3

Effects of age on Drosophila metabolism. Flies were raised on ΔS food (top panels) or ΔY food (lower panels). At the indicated ages, flies were weighed and their glycogen levels determined in triplicate. Glycogen levels decrease with age in both ΔS and ΔY treatment groups (2-way ANOVA, p < 0.0001 for the effect of age on glucose levels for either diet). On ΔS diets, overfeeding only had a small effect on glycogen levels (2-way ANOVA, p = 0.0559 for the comparison of age-dependent variation between different food levels), while overfeeding on a ΔY diet significantly reduced glycogen levels compared to underfeeding diets (2-way ANOVA, p < 0.0001). Flies raised on a slight underfeeding diet (5%) are in black, flies on an overfeeding diet (30%) are in grey. Shown are representatives of two independent experiments.