Fig. 2.

Overexpression of STIM1 leads to increased store-operated Ca²⁺ entry. (A) Representative traces of Tg-induced Ca²⁺ release and Ca²⁺ entry in L1-control and L1-STIM1 C7 cell lines. Tg and Ca²⁺ were introduced at the times indicated by the bars above the graph. (B) L1-STIM1 C7 cells were preincubated for 10 min with 3 µM BTP2 prior to stimulation with 1 µM Tg in Ca²⁺-free media followed by reintroduction of 1 mM Ca²⁺. Data represents the maximum level of store-operated Ca²⁺ entry in L1-STIM1 C7 cells treated with BTP2 or vehicle, expressed as a percentage of the vehicle control. (C, D) Representative traces of UTP-induced Ca²⁺ release and Ca²⁺ entry in L1-control (C) and L1-STIM1 C7 (D) cells. UTP and Ca²⁺ were introduced at the times indicated by the bars above the graphs, *p < 0.0001.