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. Author manuscript; available in PMC: 2013 Mar 19.
Published in final edited form as: Differentiation. 2008 Dec 2;77(3):239–247. doi: 10.1016/j.diff.2008.10.013

Fig. 5.

Fig. 5

Stim1 downregulation enhances adipogenesis in 3T3-L1 cells and leads to upregulation of C/EBPα and Adpn expression. (A) Oil Red O staining of 3T3-L1 cells at day 8 following transfection with standard control Stealth™ siRNA duplexes or Stim1–60 Stealth™ siRNA duplexes and induced to differentiate with either MDI, or 0.5MD. (B) Quantification at OD520 of lipid extracted from triplicate wells of 3T3-L1 cells at day 8. Cells were transfected with standard control, Stim1–60 or Stim1–61 Stealth™ siRNA duplexes and induced to differentiate with 0.5MD. (C) Relative Stim1 mRNA levels in Stim1 or control siRNA transfected 3T3-L1 cells harvested 48 h post transfection. Western blotting for (D) Stim1, PPARγ1 and γ2, β-actin and (E) C/EBPα, Adpn, β-actin in cell lysates collected from Stealth™ Stim1-60 (S1–60) or control (Con) siRNA duplex-transfected cells at 4 days post 0.5MD treatment.