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. Author manuscript; available in PMC: 2013 Mar 19.
Published in final edited form as: Science. 2010 Oct 1;330(6000):105–109. doi: 10.1126/science.1191086

Fig. 3.

Fig. 3

Interactions between STIM1 and CaV1.2 α1C, and store-dependent co-localization of STIM1, CaV1.2, and Orai1 proteins. (A) HEK293 cells coexpressing mCherry-STIM1 and GFP-α1C; whole-cell lysates (left) and antibody to STIM1 immunoprecipitates were probed with antibody to GFP and antibody to STIM1. (B) HEK293 cells coexpressing GFP-α1C and STIM1; whole-cell lysates (left) and antibody to GFP immunoprecipitates were probed with antibody to GFP and antibody to STIM1. (C to O) High-resolution imaging focused on the ER-PM interface in HEK293 cells. (C to H) Distribution of expressed mCherry-STIM1 and GFP-α1C (coexpressed with β2a + α2δ1 subunits), examined in cells either just before (C and E) or 5 min after (F to H) Ca2+ store depletion with 2 μM ionomycin. (I to K) Magnification of the STIM, α1C, and merged images, respectively. (L and M) Distribution of coexpressed mCherry-Orai1 and GFP-α1C (with β2a + α2δ1 subunits) in cells 5 min after ionomycin-induced emptying. (O) Magnified area of the merged image from (N).