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. 2013 Mar 19;8(3):e59369. doi: 10.1371/journal.pone.0059369

Figure 1. BCL11B is up-regulated by EWS/FLI in Ewing sarcoma cells.

Figure 1

A. A673 and TC71 Ewing sarcoma cells were infected with a control shRNA (Luc) or an shRNA targeting EWS/FLI followed by rescue with an empty vector or EWS/FLI cDNA. BCL11B RNA levels were determined by qRT-PCR. Error bars represent standard deviation (SD) of three technical replicates. P-values were determined using a Student’s T-test comparing all conditions to control (Vector+Luc shRNA) (** for p≤0.01, *** for p≤0.001). B. A673 and TC71 cells were infected as in panel A, and protein extracts were Western blotted for BCL11B and EWS/FLI. Tubulin was used as a loading control. C. A673 cells were infected with a control shRNA or shRNA targeting EWS/FLI followed by rescue with EWS/FLI cDNA, or mutants of EWS/FLI containing a DNA biding mutation (R2L2) or an EWS deletion (Δ22). Error bars represent SD of three technical replicates. P-values were determined using a Student’s T-test comparing all conditions to control (Vector+Luc shRNA) (** for p≤0.01, *** for p≤0.001). D. Western blot analysis of BCL11B levels in the same samples shown in panel C. Tubulin was used as a loading control.