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. 2013 Mar 19;8(3):e59369. doi: 10.1371/journal.pone.0059369

Figure 2. BCL11B is necessary for the maintenance of transformation in Ewing sarcoma cells.

Figure 2

A. A673 and TC71 Ewing sarcoma cells were infected with retroviral shRNA constructs targeting BCL11B (BCL11B-4 and BCL11B-6 shRNA), or luciferase (Luc) as a control. BCL11B levels were determined by western blot. Tubulin was used as a loading control. B. Growth rates of A673 and TC71 cells harboring the indicated shRNA retroviral constructs were determined using a 3T5 assay [34]. P-values were determined using a Student’s T-test comparing all conditions to control (Luc shRNA) (* for p≤0.05). C. Anchorage independent growth of control (Luc shRNA) and BCL11B (BCL11B-4 and BCL11B-6 shRNA) knock-down A673 and TC71 cells was assessed by the ability to form colonies in methylcellulose. Error bars represent SD of two technical replicates. P-values were determined using a Student’s T-test comparing all conditions to control (Luc shRNA) (* for p≤0.05, *** for p≤0.001).