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. 2013 Mar 19;8(3):e59369. doi: 10.1371/journal.pone.0059369

Figure 4. BCL11B mediates repression in Ewing sarcoma cells.

Figure 4

A. BCL11B RNA-seq results were confirmed by performing qRT-PCR for the indicated mRNAs on control or BCL11B siRNA transfected A673 cells 48 hours post-transfection. P-values were determined using a Student’s T-test comparing all conditions to control (* for p≤0.05, ** for p≤0.01). B. EWS/FLI was knocked-down using shRNA and rescued with an empty vector or BCL11B cDNA. mRNA levels of EWS/FLI and BCL11B were confirmed by qRT-PCR, and transcript levels of the indicated genes were assessed by qRT-PCR. Error bars represent SD of three technical replicates. P-values were determined using a Student’s T-test comparing all conditions to control (Luc shRNA+Vector) (left panel) or comparing conditions as indicated (right panel) (ns for not significant, * for p≤0.05, ** for p≤0.01, *** for p≤0.001).