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. 2013 Mar 19;11(3):e1001512. doi: 10.1371/journal.pbio.1001512

Figure 2. Activity of Phr peptides on RapJ phosphatase activity in vivo and in vitro.

Figure 2

(A) RapJ activity was measured as a function of PspoIIG::luc expression in strain VP068. Each curve is representative of at least three independent experiments performed in duplicate. RapJ overexpression was controlled by the IPTG-inducible promoter Phyperspank (Phs), and the oligopeptides were used at 530 µM. RLU, Relative Luminescence Units. (B) The ability of RapJ to dephosphorylate Spo0F∼P in the presence and absence of Phr peptides was compared. RapJ was used at 6.5 µM, and the Phr oligopeptides were used at 310 µM. The gels are representative of experiments repeated at least three times.