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RapI dephosphorylates Spo0F (top panel), and RapI Spo0F phosphatase activity is inhibited by the pentapeptide DRVGA (middle panel) and hexapeptide ADRVGA (bottom panel). RapI was used at 6.5 µM, and the Phr oligopeptides were used at 32.5 µM. The gels are representative of phosphatase assays repeated at least three times.
