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. 2012 Aug 29;10:51. doi: 10.1186/1477-5956-10-51

Table 1.

Proteins result analyzed and identified by MALDI-TOF-TOF/MS

Spot no Protein name Accession no. Protein MW/PI Pep. count Proten score Ratio ‘Zaosu’/the red mutant Relative protein content
Photosynthetic and energy metabolism proteins
  
  D6
 ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit
 gi|15148414
 52493.4/6.32
 21
 702
 2.57
graphic file with name 1477-5956-10-51-i1.gif
  D7
 ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit Precursor
 gi|7008057
 53067.6/6.04
 14
 520
 1.62
graphic file with name 1477-5956-10-51-i2.gif
  D9
 LHCII type I chlorophyll a/b binding protein
 gi|8954293
 27900.1/5.29
 3
 102
 0.47
graphic file with name 1477-5956-10-51-i3.gif
  D13
 Ribìlose bisphosphate carboxylase/ oxygenase activase
 gi|10720248
 48341.7/8.19
 7
 322
 5.97
graphic file with name 1477-5956-10-51-i4.gif
  D17
 oxygen-evolving complex protein 1
 gi|739292
 26603.5/5.13
 11
 576
 0.42
graphic file with name 1477-5956-10-51-i5.gif
  D18
 NAD-dependent malate dehydrogenase
 gi|15982948
 35817.5/6.6
 8
 255
 0.49
graphic file with name 1477-5956-10-51-i6.gif
  E1
 ribìlose1,5-bisphosphate carboxylase
 gi|9909955
 52296.2/6.04
 10
 305
 2.60
graphic file with name 1477-5956-10-51-i7.gif
Stress-related proteins
  
  
  D4
 Heat shock protein 70 [Cucumis sativus]
 gi|1143427
 75480/5.15
 23
 850
 0.51
graphic file with name 1477-5956-10-51-i8.gif
  D14
 shock 70 kDa protein, mitochondrial
 gi|399940
 72720.6/5.95
 12
 98
 0.37
graphic file with name 1477-5956-10-51-i9.gif
  D19
 high molecìlar weight heat shock protein
 gi|6969976
 71570.4/5.17
 24
 743
 0.37
graphic file with name 1477-5956-10-51-i10.gif
  E2
 Heat shock cognate 70 kDa protein 2
 gi|123620
 71062/5.08
 22
 555
 3.47
graphic file with name 1477-5956-10-51-i11.gif
Disease resistance-related proteins
  
  
  D16
 Pbzs315 SSH cDNA library of pear cv. ‘Zaosu’ inocìlated with Venturia nashicola Pyrus x bretschneideri cDNA, mRNA sequence
 gi|255987829
 35747.7/9.88
 6
 279
 0.33
graphic file with name 1477-5956-10-51-i12.gif
  D20
 polyphenol oxidase precursor
 gi|3282505
 67432.9/6.39
 4
 273
 0.47
graphic file with name 1477-5956-10-51-i13.gif
  D21
 S-adenosysl-L-methionine:caffeic acid3-O-methyltransferase
 gi|3913295
 40135.3/5.52
 7
 247
 0.38
graphic file with name 1477-5956-10-51-i14.gif
  D24
 polyphenol oxidase 2 precursor
 gi|14194273
 65762/6.09
 7
 178
 1.72
graphic file with name 1477-5956-10-51-i15.gif
Cytoskeleton-related proteins
  
  
  D15
 Os03g0718100
 gi|115454971
 42014/5.3
 14
 255
 Only observed in the red mutant
graphic file with name 1477-5956-10-51-i16.gif
  D22
 actin
 gi|32186902
 42057.1/5.3
 16
 531
 Only observed in the red mutant
graphic file with name 1477-5956-10-51-i17.gif
Amino acid metabolism-related proteins
  
  
  D23
 S-adenosylmethionine synthase
 gi|75308025
 43564.9/5.5
 14
 635
 0.53
graphic file with name 1477-5956-10-51-i18.gif
  E4
 putative methionine synthase
 gi|14532772
 84899.8/6.09
 12
 148
 Only observed in ‘Zaosu’ pear
graphic file with name 1477-5956-10-51-i19.gif
Antioxidant-related proteins
  
  
  D11
 Putative pyridoxine biosynthesis protein isoform A
 gi|46399269
 33353.1/5.93
 8
 218
 0.24
graphic file with name 1477-5956-10-51-i20.gif
Calcium-related proteins
  
  
  D10
 Calreticìlin
 gi|11131904
 48557.5/4.4
 14
 380
 0.49
graphic file with name 1477-5956-10-51-i21.gif
Others
  
  
  E3 Ipa. ADAB-aaa60g07.b1 IPA1-seed Arachis ipaensis cDNA 5', mRNA sequence gi|296593933 23794.4/9.29 11 92 1.60
graphic file with name 1477-5956-10-51-i22.gif