Table 7.
RT-PCR determination of gene expression in the choroid plexus
| |
3 hr |
1 day |
|||
|---|---|---|---|---|---|
| NCBI gene symbol | Control | AMPH | EIH | AMPH | EIH |
|
Apold1(Verge)a |
0.03 ± 0.002 |
0.06 ± 0.02 (2.00) |
0.06 ± 0.07 (2.00) |
N.D. |
N.D. |
|
Bmp4a,c |
0.55 ± 0.04 |
3.65 ± 1.26 (6.64) |
0.84 ± 0.23 (1.53) |
N.D. |
N.D. |
|
Cebpda,b |
0.01 ± 0.003 |
0.24 ± 0.09 (24.00) |
0.15 ± 0.13 (15.00) |
N.D. |
N.D. |
|
Cldn1 |
5.74 ± 0.70 |
5.73 ± 0.98 (1.00) |
6.49 ± 0.80 (1.13) |
N.D. |
N.D. |
|
Cxcl1a,b |
0.004 ± 0.002 |
0.40 ± 0.62 (100.00) |
0.30 ± 0.51 (75.00) |
N.D. |
N.D. |
|
Cyr61a,b |
0.24 ± 0.06 |
3.54 ± 2.70 (14.75) |
3.16 ± 5.10 (13.17) |
N.D. |
N.D. |
|
Dio2a |
0.01 ± 0.005 |
4.34 ± 2.03 (434.00) |
1.08 ± 1.13 (108.00) |
N.D. |
N.D. |
|
Edn1b |
0.003 ± 0.001 |
0.005 ± 0.002 (1.67) |
0.011 ± 0.010 (3.67) |
0.003 ± 0.004 (1.50) |
0.002 ± 0.000 |
|
Fstb,d |
0.03 ± 0.02 |
0.06 ± 0.02 (2.00) |
0.10 ± 0.06 (3.33) |
0.03 ± 0.02 (3.00) |
0.01 ± 0.003 |
|
Has1a,b |
0.09 ± 0.06 |
0.17 ± 0.03 (1.89) |
0.17 ± 0.02 (1.89) |
0.11 ± 0.02 (1.10) |
0.10 ± 0.02 |
|
Lbpa |
0.11 ± 0.06 |
0.53 ± 0.32 (4.81) |
0.24 ± 0.12 (2.18) |
0.97 ± 0.57 (2.11) |
0.46 ± 0.31 |
|
Nat3b |
0.01 ± 0.004 |
0.01 ± 0.004 (1.00) |
0.01 ± 0.008 (1.00) |
0.004 ± 0.003 (0.50) |
0.008 ± 0.010 |
|
Nos3 |
0.06 ± 0.03 |
0.09 ± 0.04 (1.50) |
0.10 ± 0.06 (1.67) |
0.08 ± 0.05 (1.33) |
0.06 ± 0.01 |
|
Oclna |
4.96 ± 0.52 |
7.24 ± 1.12 (1.46) |
6.01 ± 1.76 (1.21) |
N.D. |
N.D. |
|
Reg3b |
N.E. |
N.E. |
N.E. |
N.E. |
N.E. |
|
Sct |
N.E. |
N.E. |
N.E. |
N.E. |
N.E. |
| Sectm1ba | 0.01 ± 0.002 | 0.46 ± 0.15 (46.00) | 0.15 ± 0.14 (15.00) | N.D. | N.D. |
All genes are expressed as a percentage of Gapdh, and are shown as the mean plus/minus standard error of the mean. Fold-changes of treatment to control are given in parentheses at the 3 hr time point. At the 1 day time point, fold-changes (in parentheses) are expressed as AMPH to EIH. The number of RNA aliquots (two to three animals per aliquot) used to determine expression per treatment group were as follows: 3 hr control, n = 7; 3 hr AMPH, n = 7; 3 hr EIH, n = 7; 1 day AMPH, n = 8; 1 day EIH, n = 7.
N.E. means gene not expressed (mean Ct value > 35).
N.D. designates expression not determined.
Symbols indicating statistically significant difference between treatments are found as a superscript after the gene name (first column).
a AMPH significantly differs from control at 3 hr (p < 0.05).
b EIH significantly differs from control at 3 hr (p < 0.05).
c AMPH significantly differs from EIH at 3 hr (p < 0.05).
d AMPH significantly differs from EIH at 1 day (p < 0.05).