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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Horm Behav. 2013 Jan 28;63(3):518–526. doi: 10.1016/j.yhbeh.2013.01.005

Figure 4. Analysis of V1aR knockdown, OTR and GFP expression in shRNA-pvAvpr1a and scrambled injected male prairie voles.

Figure 4

Representative V1aR autoradiograms display a reduction in binding in shRNA-injected animals as compared to control, scramble injected animals (A, B). The square surrounding the ventral pallidum (VP) represents the location of injection sites (reprinted from Paxinos and Watson, 1998) (C). V1aR expression was reduced by 30% in shRNA-injected subjects as compared to controls (D). OTR expression in the nucleus accumbens was unchanged, as seen in representative autoradiograms (E, F). GFP immunoreactivity at the injection site displays typical viral spread (10X, G) and clear expression in neuronal cell bodies and processes (20X, H). Scale bars are 1mm in F and 50μm in G, H. Asterisk indicates p-value <0.05.