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. 2013 Mar 19;8(3):e59307. doi: 10.1371/journal.pone.0059307

Figure 6. Dose-dependent ISG-inducing potency of ChIFN-α and ChIFN-β.

Figure 6

After DF-1 cells were incubated with 2, 10, and 50 U/ml ChIFN-α or ChIFN-β, the cells were harvested at 12 h post treatment for RNA extraction and cDNA preparation. The mRNA levels of 2′,5′-OAS (A), PKR (B), STAT1 (C), and MyD88 (D) were assayed by real time PCR. Data were shown as mean ± SEM (n = 3). The Mann-Whitney U test was used to compare the differences in relative mRNA levels between ChIFN-α and ChIFN-β treatments at the same time points. A value of P<0.05 was considered statistically significant. *P<0.05 and **P<0.01. The asterisks were masked on the top of higher columns.