Figure 2.

Transglutaminase 2 (TG2) deficiency reduces eosinophil recruitment. (A–C) Bronchoalveolar lavage (BAL) fluid from WT and TG2^−/− mice was obtained 48 h after the last OVA challenge. BAL cells were counted and analyzed by Wright-Giemsa staining. Total (A) and differential cell counts (B) were performed. (C) Representative photographs of BAL cells. Cells were stained with Wright–Giemsa (original magnification, ×1000). Arrows present eosinophils. (D) Inflammatory cells in BAL fluid from WT and TG2^−/− mice were labeled with anti-CCR3 and anti-Siglec-F antibodies and analyzed by flow cytometry. (A-B) Data are means ± standard deviation of three independent determinations with BAL cells from n = 5 mice/group.