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MG63 osteosarcoma cells were treated with vehicle (Veh), 10 µM 2-ME, 2-ME plus 100 nM ATG7siRNA (ATG7Si) and 2-ME plus 100 nM Non specific siRNA (NSsi) for 48 hrs and analyzed by western blot hybridization using anti-ATG7 and anti-actin antibodies.
