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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Neurosci Methods. 2013 Feb 4;214(2):149–155. doi: 10.1016/j.jneumeth.2013.01.024

Figure 1. Strong ChR2 expression in the terminal fields of the striatum.

Figure 1

ChR2 expression in the terminal fields of the striatum. 400 micron-thick coronal slices were obtained and quickly visualized while floating in aCSF using a fluorescent stereomicroscope prior to voltammetric analysis. Top panels show two locations (A and B) in the striatum. Bottom panels demonstrate expression of the ChR2-EYFP fusion proteins in the terminals as assessed by native fluorescence in approximately the same locations (C and D, respectively). The monochromatic images were pseudocolored to enhance contrast, and the scale indicates relative intensity of the EYFP signal.