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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Immunol. 2013 Feb 13;54(3-4):435–442. doi: 10.1016/j.molimm.2013.01.006

Figure 3. Opposing regulation of miR-21 by Bcl6 and Stat3.

Figure 3

A. QPCR analysis of expression of miRs – 21, 22 and 146b following ectopic expression of Bcl6 in naïve T cells. Sorted RV+ T cells were re-stimulated for 6 hrs to assess its effect on miR expression. Data are averaged from at least 3 different experiments.

B. Luciferase activity in Jurkat T cells co-transfected with full-length or SB1 and SB2 mutated miR-21 promoter driven luciferase reporters and expression constructs for CXN and CXN-Bcl6. Cells were stimulated with PMA and Ionomycin for 24 hrs prior to harvest and luciferase measurement. Results are averaged from 5 independent experiments, where the basal luciferase activity of each promoter construct was set to 100%.

C. QPCR analysis of miR-21 expression following culture of WT, Bcl6−/− and Stat3−/− naïve T cells (CD4+CD62L+) in response to increasing doses of IL-6 (0, 0.1, 1, 10 ng/ml) for 24 hrs, with expression normalized to U6. Data representative of 2 independent experiments.

(A–B) *p<0.05, **p<0.01 (Student’s t-test) (error bars=s.e.m.)