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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2013 Feb 1;376(2):245–259. doi: 10.1016/j.ydbio.2013.01.022

Figure 6. Org-1 is required for proper anteroposterior patterning of the developing visceral musculature.

Figure 6

(A) Sex combs reduced (Scr) expression is initiated in the visceral musculature (visualized by Fasciclin III (FasIII) staining) of stage 13 embryos in parasegment (PS) 4 (asterisk). (B) In org-1 mutant background visceral mesodermal Scr expression is nearly completely abolished. (C) Visceral muscle specific odd-paired (opa) expression can be detected in PS5 (asterisk) and PS10-12 (bracket). (D) In an org-1 mutant embryo both expression domains are absent. (E) Teashirt (Tsh) shows two expression domains in the visceral musculature: one in PS4-6 (bracket) and another one in PS8 (asterisk). (F) Loss of Org-1 expression leads to abolishment of both expression domains of visceral mesodermal Tsh. (G) Stage 13 wildtype embryo showing Ubx expression in PS7 of the developing visceral musculature (bracket). (H) In the org-1OJ487 genetic background Ubx expression in PS7 is completely abolished. (I) Dpp shows strong expression domains in PS3 (asterisk) and PS6-7 (bracket) of the developing visceral musculature. (J) Only weak dpp expression can be detected in PS3 and PS7 in the org-1 mutant background. (K) In a stage 13 wildtype embryo, Wg can be detected in PS8 (bracket) of the developing visceral musculature. (L) In an org-1 mutant embryo Wg expression can no longer be detected in PS8. (M) In a stage 13 embryo pointed (pnt) transcript is detectable in PS8-9 of the developing visceral musculature (bracket) and the trachea adjacent to it (tr). (N) In an org-1 mutant embryo the visceral musculature specific expression of pnt is not present. (O) Overexpression of Wg in the whole visceral mesoderm of wildtype embryos via bap-Gal4 induces between PS4-6 (bracket) and PS8 (asterisk) a strong ectopic Tsh expression domain (arrowhead). (P) In an org-1OJ487 embryo, forced induction of Wg expression in the visceral mesoderm causes only faint Tsh expression anterior to PS8 (asterisk). The visceral musculature is visualized by bap3-lacZ reporter stainings (β-Gal).