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. 2013 Mar 19;8(3):e59301. doi: 10.1371/journal.pone.0059301

Figure 4. HSCARG inhibits mRNA of p47phox.

Figure 4

(A) The effect of HSCARG on mRNA level of p47phox. Various amounts of pRK-Flag-HSCARG (0, 0.5, 1.0, 2.0 µg) were transfected into HEK293 cells, and the mRNA levels of p47phox were measured by RT-PCR. Actin was used as a loading control. (B) Band intensities of p47phox mRNA level were shown, which were calculated and compared to that of non-transfected cellls. (C) Various amounts of HSCARG (0, 0.5, 1.0, 2.0 µg) were transfected into HEK293 cells, and the mRNA levels of p47phox were measured by quantitative real-time PCR. Values are mean ± S.E. of 3 different experiments (*p<0.05 vs. control, **p<0.01 vs. control).