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. 2013 Mar 19;8(3):e58511. doi: 10.1371/journal.pone.0058511

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© 2013 Im et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic drawing of the Snx5 gene-trapped (XA155) allele. Arrows indicate the genotyping primers, and the 2 arrowheads indicate the ER1 (EcoRI) digestion sites. The red bar represents the probe for Southern blotting. (B) Whole-embryo X-gal staining at E11.5. Scale bars: 2.5 mm. (C) Western blot analysis of the indicated mouse tissues (Br, brain; T, thymus; L, lung; S, stomach; Sp, spleen; K, kidney; In, intestine; Sk. skin; Te, testis; F, fat; U, uterus). (D) Survival rate of mice after birth. The asterisk shows the dramatic mortality observed in the neonatal stage.