Figure 2. RelB/NF-κB2 intrinsically regulate HSPC self-renewal.

(A) Limiting-dilution competitive repopulation analysis of control (Con) and dKO mice. Five to eight mice were transplanted at each dose (10, 50, 100 and 500 KLS cells/recipient for both Con and dKO groups). Peripheral blood cells of the recipients were analyzed 16 weeks after transplantation and recipient mice containing less than 1% donor cells in any one lineage were scored as negative. (B) Engraftment of control and dKO (3 × 10⁷ whole bone marrow cells/recipient). (n=7, *p<0.01). (C) Secondary bone marrow transplantation using whole bone marrow cells isolated from primary recipients transplanted with control or dKO cells as in (B). (n=6, *p<0.01). (D) dKO and control bone marrow cells have equivalent homing ability. 2×10⁶ whole bone marrow cells from control or dKO mice were transplanted into lethally irradiated recipients. Data are chimerism after 20 hours (n=3 for control and 4 for dKO). (E) Kinetic lineage contribution of dKO cells (gated on CD45.2⁺CD45.1⁻) after transplantation (n=3 at 4 weeks and n=2 at 8 and 16 weeks). Values are means +/− sem.