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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2013 Feb 4;376(2):136–149. doi: 10.1016/j.ydbio.2013.01.026

Fig 1. HIF-1α expression in the epicardium and subepicardium.

Fig 1

Costaining for HIF-1α and the myocardium marker MF20 reveals the HIF-1α staining in the epicardium and subepicardium during stage HH24-28. HIF-1α expression was observed in a few ventricular epicardial cells at stage HH24 (A–D) and stage HH28 (M–P). (E–L) HIF-1α is especially intense in the epicardium and subepicardium at the AVJ at stage HH26 (E–H) and continued to be nuclear-localized in this area to stage HH28 (I–L). HIF-1α was also detected in myocardial cells (E–P). Dashed lines delineate the border between the myocardium and the epicardium/subepicardium. Arrows point to HIF-1α positive epicardial or subepicardial cells and arrowheads point to HIF-1α positive myocardial cells. Bar = 20 μm for A–H and M–P, Bar = 40 μm for I–L.