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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2013 Feb 4;376(2):136–149. doi: 10.1016/j.ydbio.2013.01.026

Fig 4. EPDCs infected with AdcaHIF1α invaded the subepicardium.

Fig 4

Cell migration into and within the sub-epicardium was not affected even within the thick subepicardium at the AVJ (A–C). (A) Total cell numbers in the epicardium and subepicardium at AVJ counted in DAPI stained sections were not significantly different between control and AdcaHIF1α infected hearts. (B, C) GFP positive cells were found in the thick sub-epicardium of the AVJ both in AdGFP and AdcaHIF1α infected quail hearts with no obvious differences in numbers or location. epi: epicardium; se: sub-epicardium. Bar = 25 μm for B and C. (D) Quantitative analysis indicated that the number of AdcaHIF1α infected cells located in the sub-epicardium of the AVJ was not significantly different from the number of AdGFP infected cells assayed at two stages, n=6. (E) Transwell experiments demonstrated that the initial response to growth factor stimuli, the release from neighboring epicardial cells and the short migration through the 8 μm pore through the 10 μm thick Transwell membrane, was not altered in epicardial cells with caHIF1α. SFM: serum free medium.