Table 3.

Pharmacokinetic parameters for GS-DCBN and other metabolites in nasal wash samples from WT mice^a

	DCBN dose (mg/kg), route	GS-DCBN	Gly-Cys-DCBN	Cys-DCBN	N-acetyl-Cys-DCBN
AUC0–24 (h*pmol/ml)	10, i.p.	29.1	1.14	8.4	10.1
	5, i.p.	14.8	0.58	2.4	3.02
	1 × 4, s.c.	6.2	0.29	3.4	3.4
t1/2 (h)	10, i.p.	3.3	3.2	6.4	7.3
	5, i.p.	4.9	4.8	13.9	2.6
	1 × 4, s.c.	4.9	3.3	12.5	11.4

^aTwo- to three-month old, male and female WT mice (n = 3–6 for each dose and time point) were treated with DCBN intraperitoneally (single injection at 5 or 10 mg/kg), or subcutaneously (four consecutive injections, each at 1 mg/kg, with 1.5-h intervals). Nasal-wash samples were collected from dissected nasal cavity of individual mice at various times (0.5–24 h) after the last dose, for LC-MS/MS analysis, as described in Materials and Methods. The mean levels of individual metabolites were used for calculation of pharmacokinetic parameters. Levels at individual time points are shown in Table 4.