Fig. 2. The accelerated tumor progression in p53^null hosts is associated with an increase in MDSCs and expansion of lymphoid-like stromal network.

(A) TIL-MDSCs and subpopulations from WT and p53^null mice were analyzed (n= 5-10). (B) Proliferating TIL-MDSCs in tumor bearing mice were analyzed as BrdU⁺CD11b⁺Gr-1⁺ cells 24 hours post-BrdU injection. (C) Representative tumor histology images from WT and p53^null mice. Scale bar = 100 mm. (D) Representative tumor IHC images (200X) from WT and p53^null mice revealing blood endothelial cells (BEC, LYVE-1⁻Gp38⁻CD31⁺), lymphatic endothelial cells (LEC, LYVE-1⁺Gp38⁺CD31⁺), fibroblastic reticular cells (FRC, ER-TR7⁺Gp38⁺α-SMA⁺), and CD11b⁺ cells. The relative area of CD31⁺ vasculatures and ER-TR7⁺ FRC-stroma was determined using a computer-assisted program. Data are mean ± s.e. (n= 3). * denotes a significant difference (p < 0.05).