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. Author manuscript; available in PMC: 2014 Apr 12.
Published in final edited form as: J Mol Biol. 2013 Jan 23;425(7):1183–1197. doi: 10.1016/j.jmb.2013.01.016

Figure 3.

Figure 3

Detailed nucleation kinetics analysis for K2Q10pGQ11K2. (a) Concentration dependent aggregation kinetics. (b) Time2 plots of the data from panel a. (c) Log-log plot of the rates from panel b vs. starting concentration for K2Q10pGQ11K2 compared to similarly obtained data 23 for K2Q23K2. (d) Determination of the equilibrium position for fibril elongation (i.e., the Cr value) from fibril association (i.e., growth) and dissociation curves. (e) Pseudo-first order elongation kinetics from a solution of 59 μM monomer seeded with ~ 12 % weight/weight amyloid. (f) Titration of the growth points on the amyloid preparation used in the panel e experiment, using biotinyl-K2Q30K2 (Methods); the plateau (~ 60 fmol) indicates the molar amount of growth sites in the sample tested.