Figure 4. Functionality of combined Trp mutations.
A, Relative resistance of mutant and control strains to 60 µM FK506. Bars indicate the mean relative growth ± SEM of triplicate samples, normalized to Wt, from five independent experiments. B, Relative mating efficiency ± SEM of five independent experiments. (*) indicate samples that are significantly different than Wt (p<0.01). N-triple mutant: W132Y/W158Y/W311Y; Quad mutant: W228F/W311Y/W694L/W1104Y.