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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 19;1828(3):1159–1168. doi: 10.1016/j.bbamem.2012.12.005

Figure 6. Drug stimulate/inhibited ATPase activity of purified Trp mutant protein.

Figure 6

Wt and mutant proteins were assayed for ATPase activity in increasing concentrations of verapamil (A), valinomycin (B), FK506 (C), and cyclosporin A with 150 µM verapamil (D). Data points indicate the average specific activity from multiple time points ± SEM from at least four independent experiments, relative to the maximum Wt activity. Where not visible, error bars are smaller than the symbols. Lines represent non-linear regression analysis of the data points with a Hill equation. R2 values for the data fits were between 0.97 and 0.99. Kinetic parameters of the data fits are given in Table 1.