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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 19;1828(3):1159–1168. doi: 10.1016/j.bbamem.2012.12.005

Figure 7. Intrinsic Trp fluorescence of Wt and mutant Pgp.

Figure 7

100 nM purified Pgp in 0.1% DDM solutions were excited at 295 nm and the fluorescent emission spectra were collected from 310–410 nm. Lines represent the average corrected emission spectra from three independent experiments normalized to the maximum emission of Wt Pgp. The emission maxima of all four proteins occurred between 325 and 228 nm. For comparison, the emission spectra of a 1µM solution of the Trp analog NATA is also shown.