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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 19;1828(3):1159–1168. doi: 10.1016/j.bbamem.2012.12.005

Table 1.

Yield and ATPase activity of Wt and Trp mutant proteins

Yield
(mg/fermentor culture) a
ATPase
Activity b
(µmole Pi mg−1 min−1)
Wt 1.3 0.49 ± 0.02
W228F/W311Y 1.5 0.54 ± 0.03
W694L/W1104Y 1.4 0.30 ± 0.01
Quad 1.1 0.63 ± 0.08
a)

A 15 liter fermentor culture routinely gives between 80 to 90 g of wet cells.

b)

Maximum drug-stimulated ATPase activity in the presence of 125 µM verapamil (mean ± SEM)