Table 1.
Yield (mg/fermentor culture) a |
ATPase Activity b (µmole Pi mg−1 min−1) |
|
---|---|---|
Wt | 1.3 | 0.49 ± 0.02 |
W228F/W311Y | 1.5 | 0.54 ± 0.03 |
W694L/W1104Y | 1.4 | 0.30 ± 0.01 |
Quad | 1.1 | 0.63 ± 0.08 |
A 15 liter fermentor culture routinely gives between 80 to 90 g of wet cells.
Maximum drug-stimulated ATPase activity in the presence of 125 µM verapamil (mean ± SEM)