Skip to main content
. 2013 Mar 19;8(3):e59057. doi: 10.1371/journal.pone.0059057

Figure 3. Ectopic XB130 expression in MRO cells reduced levels of miR-33a, miR-149 and miR-193a-3p.

Figure 3

MRO cells were transfected with GFP vector alone, or GFP-XB130, GFP-XB130ΔN (XB130 N-terminus deletion mutant). GFP positive cells were collected by FACS. (A) Western blotting revealed that MRO cells express very low XB130. Transfection of GFP-XB130 (MRO-XB130) or GFP-XB130ΔN (MRO-XB130ΔN) showed XB130 expression in MRO cells. The mature forms (B) and primary transcripts (C) of miR-33a, miR-149 and miR-193a-3p were examined by real-time quantitative RT-PCR. These miRNAs and pri-miRNAs in MRO cells were significantly higher than that in WRO cells, and significantly reduced by overexpression of GFP-XB130, but not by GFP-XB130ΔN. *P<0.05 compared with WRO cells.